phospho p38 (Cell Signaling Technology Inc)
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phospho p38
Phospho P38, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+p38+mapk/p38gamma+MAPK+Antibody/pm42892647-235-84-85
Average 94 stars, based on 52 article reviews
Phospho P38, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+p38+mapk/p38gamma+MAPK+Antibody/pm42892647-235-84-85
Average 94 stars, based on 52 article reviews
phospho p38 - by Bioz Stars,
2026-10
94/100 stars
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Incubation:Article Title: Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach Article Snippet: Equal protein amounts were denatured, separated by 12 % SDS-PAGE, and transferred onto 0.45 μm PVDF membranes (Merck-Millipore, Burlington, MA, USA). .. Membranes were blocked with 5 % non-fat milk in TBS-T and incubated overnight at 4 °C with primary antibodies against phospho-AKT (Thr308), phospho-NFκB p65 (Ser536), Article Title: Orexin‐A Inhibits Lipopolysaccharide‐Induced Cell Migration in Cultured Mouse Astrocytes via Activation of Orexin 1 Receptor: Involvement of GABA Signaling Article Snippet: .. The membrane was incubated overnight at 4°C with a primary antibody specific for OXA (1:500, cat# AB3098, EMD Millipore), OX1R (1:500, cat#PA5‐77566, Invitrogen), GAT1 (1:300, cat# ab426, Abcam), GAT3 (1:300, cat# 25438, Cell Signaling Technology), KCC2 (mouse monoclonal anti‐KCC2 antibody, 1:500, cat# MA5‐27610, Thermo Fisher Scientific), phospho‐KCC2 (rabbit polyclonal anti‐phospho‐KCC2 (Thr‐1007) antibody, 1:500, cat# PA5‐95677, Thermo Fisher Scientific), ERK (1:1000, cat#9102, Cell Signaling Technology), phospho‐ERK (1:1000, cat#9101, Cell Signaling Technology), p38 MAPK (1:1000, cat#9212, Cell Signaling Technology), Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), Electrophoresis:Article Title: Epigenetic Regulation of VCAM-1 by Lipoxin A4 Is Renoprotective Against Diabetic Kidney Disease Article Snippet: Cultured hGECs were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) with Phosphatase Inhibitor Cocktail 2 (Sigma) and Complete Protease Inhibitor Cocktail (Roche), and total protein was quantitated by bicinchoninic acid assay (Thermo Fisher Scientific). .. Following electrophoresis and transfer, the following primary antibodies were used: VCAM-1 (1:1,000; Abcam); Western Blot:Article Title: Bi-allelic EPHA2 mutations cause dysosteosclerosis by inhibiting osteoclastogenesis and enhancing osteoblastogenesis. Article Snippet: Immunohistochemistry staining was performed as described previously.48 The sections were incubated with rabbit anti-Osteocalcin antibodies (1:500; ab93876, Abcam) or anti-Sp7/ Osterix antibodies (1:500; ab209484, Abcam). .. Western blot analysis Protein extraction and western blot were performed as described previously.48 Each sample was probed for p38 MAPK (1:1 000 dilution, 9212, CST), Analysis:Article Title: Bi-allelic EPHA2 mutations cause dysosteosclerosis by inhibiting osteoclastogenesis and enhancing osteoblastogenesis. Article Snippet: Immunohistochemistry staining was performed as described previously.48 The sections were incubated with rabbit anti-Osteocalcin antibodies (1:500; ab93876, Abcam) or anti-Sp7/ Osterix antibodies (1:500; ab209484, Abcam). .. Western blot analysis Protein extraction and western blot were performed as described previously.48 Each sample was probed for p38 MAPK (1:1 000 dilution, 9212, CST), Protein Extraction:Article Title: Bi-allelic EPHA2 mutations cause dysosteosclerosis by inhibiting osteoclastogenesis and enhancing osteoblastogenesis. Article Snippet: Immunohistochemistry staining was performed as described previously.48 The sections were incubated with rabbit anti-Osteocalcin antibodies (1:500; ab93876, Abcam) or anti-Sp7/ Osterix antibodies (1:500; ab209484, Abcam). .. Western blot analysis Protein extraction and western blot were performed as described previously.48 Each sample was probed for p38 MAPK (1:1 000 dilution, 9212, CST), Membrane:Article Title: Orexin‐A Inhibits Lipopolysaccharide‐Induced Cell Migration in Cultured Mouse Astrocytes via Activation of Orexin 1 Receptor: Involvement of GABA Signaling Article Snippet: .. The membrane was incubated overnight at 4°C with a primary antibody specific for OXA (1:500, cat# AB3098, EMD Millipore), OX1R (1:500, cat#PA5‐77566, Invitrogen), GAT1 (1:300, cat# ab426, Abcam), GAT3 (1:300, cat# 25438, Cell Signaling Technology), KCC2 (mouse monoclonal anti‐KCC2 antibody, 1:500, cat# MA5‐27610, Thermo Fisher Scientific), phospho‐KCC2 (rabbit polyclonal anti‐phospho‐KCC2 (Thr‐1007) antibody, 1:500, cat# PA5‐95677, Thermo Fisher Scientific), ERK (1:1000, cat#9102, Cell Signaling Technology), phospho‐ERK (1:1000, cat#9101, Cell Signaling Technology), p38 MAPK (1:1000, cat#9212, Cell Signaling Technology), other:Article Title: Hemoglobin inhibits fibroblast-to-cardiomyocyte reprogramming via TLR2/TLR4-dependent chromatin compaction Article Snippet: Band intensities were analyzed with either ImageJ or Syngene software. Hemagglutination Assay:Article Title: SIK2 antagonizes postinfarction cardiac repair by driving macrophage proinflammatory and impairing efferocytosis Article Snippet: .. Primary antibodies used to specifically detect the proteins of interest were as follows: SIK2 (6919s, CST), GAPDH (G9545, Sigma-Aldrich), α-SMA (A5228, Sigma-Aldrich), Collagen I (AB765P, Sigma-Aldrich), Collagen III (AB7778, Abcam), CD31 (3528s, CST), Phospho-NF-κB P65 (Ser 536 ) (3033s, CST), NF-κB P65 (8242s, CST), Nonspecific Binding:Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), Cell-Signaling:Article Title: Docetaxel treatment upregulates estrogen receptor expression in triple negative breast cancer cells via activating arachidonic acid pathway Article Snippet: .. The membranes were blocked with 3% bovine serum albumin (BSA) to prevent nonspecific binding and incubated with primary antibodies: anti-Estrogen Receptor alpha antibody (ab75635, Abcam), Cleaved caspase 3 (9661, Cell Signaling Technology, CST), c-Jun (9165, CST), Phospho-c-Jun (3270, CST), Phospho-SAPK/JNK (4668, CST), |
![( A ) KEGG enrichment analysis of down-regulated DEGs in Sik2 F/F ; LysM Cre cardiac macrophages on day 3 post-MI. The top 20 enriched pathways are listed. ( B ) DEGs (fold change ≥ 2 and P .adjust ≤ 0.05) overlapped in top 20 of enriched pathways. ( C ) Transcription factor prediction was performed using TRRUST databases. The top 10 transcription factors are listed. ( D ) Immunoblotting analysis and quantification of P65 in the cytoplasm and nucleus of WT and SIK2-knockout PMs and treated with TNF-α (20 ng/ml) or vehicle ( n = 4) for 1 hour. ( E ) qPCR analysis of Il-1β , Il-6 , Nos2 , Cxcl-1 , and Mmp-9 mRNA expression in WT and SIK2-knockout PMs stimulated with TNF-α for 24 hours ( n = 6). ( F ) qPCR analysis of Il-1β , Il-6 , Nos2 , Cxcl-1 , and Mmp-9 mRNA expression ( n = 6). PMs were pretreated with ARN3236 (3 μmol) and stimulated with TNF-α for 24 hours. ( G ) WikiPathways enrichment analysis of differentially phosphorylated proteins in PMs treated with ARN3236 (3 μmol) or control. The top 20 enriched pathways are listed. ( H ) Immunoblotting analysis and quantification of phosphorylated P65 (p-P65), P65, phosphorylated <t>P38</t> <t>(p-P38),</t> and P38 expression in WT and SIK2-knockout PMs stimulated with TNF-α or vehicle ( n = 6). ( I and J ) Flow cytometry analysis of efferocytosis in WT or LeSik2 Raw264.7 cells [stimulated with SB 203580 (10 μmol) or not] treated with CFSE-prelabeled RBCs ( n = 6).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4512/pmc13674512/pmc13674512__sciadv.adw6624-f5.jpg)
